Modifying Enzymes
E. coli Pyrophosphatase (PPase), 50 U, G-Biosciences
E. coli Inorganic Pyrophosphatase (PPase) speeds up the breakdown of inorganic pyrophosphate (PPi) into orthophosphate.
T4 RNA ligase II, 750 U, G-Biosciences
T4 RNA Ligase II (gp24.1) repairs nicks in dsRNA and can also be used to ligate the 3' terminal OH of RNA to the 5'-terminal phosphate of DNA in a double-stranded structure.
T3 DNA Ligase, 750000 U, G-Biosciences
T3 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5' phosphate and a 3' hydroxyl termini in duplex DNA; joining blunt and cohesive end termini as well as repairing single stranded nicks in duplex DNA.
G-Biosciences T5 Exonuclease
T5 Exonuclease is a highly efficient 5'-to-3' exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions.
LGC GENOMICS LLC (Lucigen) Ampligase DNA Ligase with Buffer, 750 U @ 5 U/LHighly thermostable DNA ligase for applications where ligation at high temperature is beneficial or required.
Ampligase Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3-hydroxylated and 5-phosphorylated termini in duplex DNA structures that are stable at high temperatures
New England Biolabs, Inc. EcoRI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^AATT_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. Proteinase K, Molecular Biology Grade – 2 x 1 ml vials
Proteinase K is a subtilisin-related serine protease that hydrolyzes a variety of peptide bonds and is frequently used to cleanup enzymatic reactions or cell lysates.
Innovative Cell Technologies ACCUTASE CELL DETACHMENT REAGN
Accutase; Sterile; 100mL Enzyme cell detachment medium; Composition: Proprietary formulation of enzymes, water, phosphate buffered saline, phenol red; Stability: Stable under normal temperatures and pressures
Sigma Aldrich Fine Chemicals Biosciences DNASE I GRADE II, FROM BOVINE
DNase I grade II, from bovine pancreas 100mg
FUJIFILM BIOSCIENCES INC Lysyl Endopeptidase, Mass Spectrometry Grade (Lys-C), 5 x20UG
*Orders for this supplier are non-cancellable/non-returnable once they are processed.* Lysyl Endopeptidase, Mass Spectrometry Grade. For Proteome Research. 20 ug x 5 vials.
LGC GENOMICS LLC (Lucigen) Proteinase K, 2 mLDigest proteins during DNA and RNA purification or inactivate enzymes. To be used with the MasterPure Kits.
This reagent is designed for use with the MasterPure™ kit protocols only.